My eyes were bigger than my brain. I had big ideas about knocking out two big molecular biology labs last week. It turns out we can succesfully exectute and understanf one big laboratory activity a week.
This week we will get into one my favorite labs in the curriculum, DNA mediated transformation. That’s a fancy way of saying we’re gonna stick a jelly fish gene into E. coli and induce them to glow green. The mechanics of this lab are quite simple, but the science behind it complicated and fascinating . Come prepared to work, take notes and think. I’m looking forward to it.
Monday (11/2/09): PCR of PV92 Labs Due.
Monday (11/2/09): Transformation of E. coli with pGLO plasmid, Day 1
Tuesday (11/3/09): data collection for transformation lab (introduction to operons)
Wednesday (11/4/09): More Central Dogma Lectures: Mutations, and microbial genetics, more on Operons
Thursday (11/5/09): Thursday: 45 minuteswork day on pGLO lab, mutation assignment, 45 Minutes: operon models, eukaryotic genomes
Thursday (11/5/09): Ken Miller speaks at UAB Honors House: “Teaching Evolution in America”7pm, Open to the public
Friday (11/6/09) Review of chapters 16-19
HEADS UP: Molecular Biology Exam: Thursday (11/12/09)
Tags: central dogma, lab work, molecular biology, pGLO, Week 13
